The Microbial Genome Database for Comparative Analysis is constantly growing with the addition of newly sequenced genomes.
How MBGD Is Used Edit
In this database, the similarities between genes are already entered and stored into the database. The genes that have been previously added also include the function they have in the cell through their translated sequences. "Using homology data, MBGD dynamically creates orthologous gene cluster table. Users can change a set of organisms or cutoff parameters to create their own orthologous grouping. Based on this cluster table, users can further analyze multiple genomes from various points of view with the functions such as global map comparison, local map comparison, multiple sequence alignment and phylogenetic tree construction (1)."
How To SearchEdit
When first entering the site, there is a table containing all of the organisms that the MBGD consists of. The organism(s) of choice must be selected. From the main page, there are a multitude of ways that
one can search: keyword search, advanced search, homology search, and map search. The "keyword search" option searches for the entered word against the database. The "advanced search" option allows the user to narrow down the search by including specific keywords for each field. The "homology search" matches the sequences of query against protein sequences in the database. The "map search" enables the user to choose a particular gene from a chromosome map. Searching MBGD through a keyword search first consists of typing in the keyword of choice into the box (View Picture 1).Once the keyword of choice is selected and the search has begun, the results based on the entered keyword will show (View Picture 2).
The View Homology Cluster Table can be clicked to see a cluster table with the retrieved genes(Highlighted in red: Picture 3). The desired gene can be chosen and the information on said gene will then project including its description, name, function, organism it is found in, and position.
"Welcome to MBGD." Microbial Genome Database. JSPS, NBDC, and JST, n.d. Web. 8 Sept. 2013. <mbgd.genome.ad.jp>.