High-throughout sequencing is an umbrella term that is used to group high-efficiency methodologies for replicating DNA such as Massively parallel signature sequencing, Polony sequencing, 454 pyrosequencing (etc.) It is sometimesl referred to as massively parallel DNA sequencing, because thousands or millions of sequencing reactions are run at once. This practice differs from Shotgun sequencing in that the DNA fragments produced contain less base pairs. Rapid development of high-throughout sequencing methods has been seen in recent years because it is a time and cost effective way to sequence and analyze large genomes.
Usual methodologies involve PCR and physical binding of template DNA to a solid surface or to tiny beads called microbeads. A single 10-hour run of a machine can produce 400 million or more base pairs of DNA sequence information.
- DNA sequencing. (2013, September 22). In Wikipedia, The Free Encyclopedia. Retrieved 00:56, October 2, 2013